Ding W, Zhang W R, Sullivan K, Hashimoto N, Goldstein B J
Dorrance H. Hamilton Research Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Biochem Biophys Res Commun. 1994 Jul 29;202(2):902-7. doi: 10.1006/bbrc.1994.2015.
Protein-tyrosine phosphatases (PTPases) are among the fastest growing family of enzymes that are closely linked to signal transduction pathways involving reversible tyrosine phosphorylation. In order to identify PTPase homologs expressed in adipocytes that might regulate the action of insulin or growth factors in this tissue, we screened a rat adipocyte cDNA library at reduced stringency with a panel of candidate PTPase probes. After subcloning and sequence analysis of the positive plaques, this approach enabled us to identify the expression of LRP/RPTP-alpha, PTPase 1B, SH-PTP2/Syp, and LAR in adipocytes at an abundance of 16, 7, 6 and 3 per million, respectively. Furthermore, a sequence variant of SH-PTP2/Syp was identified that may have significance in the tissue-specific activity of this enzyme. These data provide insight into PTPase homologs that may have a physiological role in the regulation of phosphotyrosyl turnover in hormone signalling pathways in adipocytes.
蛋白质酪氨酸磷酸酶（PTPases）是增长最快的酶家族之一，与涉及可逆酪氨酸磷酸化的信号转导途径密切相关。为了鉴定在脂肪细胞中表达的可能调节该组织中胰岛素或生长因子作用的PTPase同源物，我们用一组候选PTPase探针在较低严谨度下筛选了大鼠脂肪细胞cDNA文库。对阳性噬菌斑进行亚克隆和序列分析后，该方法使我们能够鉴定出脂肪细胞中LRP/RPTP-α、PTPase 1B、SH-PTP2/Syp和LAR的表达，其丰度分别为每百万个细胞中16、7、6和3个。此外，还鉴定出了SH-PTP2/Syp的一个序列变体，它可能在该酶的组织特异性活性中具有重要意义。这些数据为可能在脂肪细胞激素信号通路中磷酸酪氨酸周转调节中发挥生理作用的PTPase同源物提供了见解。
