Nothnick W B, Curry T E, Vernon M W
Department of Physiology, University of Kentucky, Lexington 40536-0084.
Am J Reprod Immunol. 1994 Mar-Apr;31(2-3):151-62. doi: 10.1111/j.1600-0897.1994.tb00860.x.
The immune system has been implicated in the pathophysiology of endometriosis. To determine if modulation of the immune system influences endometriotic implant growth and protein production, the following experiment was conducted.
Female rats with surgically induced endometriosis were treated with either the immunosuppressive agent pentoxifylline (Pent; 5 mg/kg BW; N = 16) or a vehicle (N = 16) for 7 consecutive days, then killed. Twenty-four hours before death, 8 animals from each group were injected intraperitoneally with the immunostimulatory agent lipopolysaccharide (LPS; 200 micrograms/kg BW). At death, endometriotic implants were measured and protein production assessed by two-dimensional polyacrylamide gel electrophoresis.
Pentoxifylline significantly (P < 0.001) reduced endometriotic implant size (2.15 +/- 0.65 mm2 vs. 17.13 +/- 1.98 mm2) whereas LPS was without effect (18.32 +/- 2.57 mm2 vs. 17.13 +/- 1.98 mm2). Pentoxifylline also suppressed production of a portion of the proteins that comprise the implant specific group of proteins, ENDO-2, whereas LPS induced the production of two additional ENDO-2 proteins.
Immunomodulatory agents can modulate rat endometriotic implant growth and production of implant-specific proteins.
免疫系统与子宫内膜异位症的病理生理机制有关。为了确定免疫系统的调节是否会影响子宫内膜异位症植入物的生长和蛋白质生成，进行了以下实验。
对通过手术诱导产生子宫内膜异位症的雌性大鼠连续7天给予免疫抑制剂己酮可可碱（Pent；5毫克/千克体重；N = 16）或赋形剂（N = 16），然后处死。在处死前24小时，每组8只动物腹腔注射免疫刺激剂脂多糖（LPS；200微克/千克体重）。处死时，测量子宫内膜异位症植入物的大小，并通过二维聚丙烯酰胺凝胶电泳评估蛋白质生成情况。
己酮可可碱显著（P < 0.001）减小了子宫内膜异位症植入物的大小（2.15 ± 0.65平方毫米对17.13 ± 1.98平方毫米），而脂多糖没有效果（18.32 ± 2.57平方毫米对17.13 ± 1.98平方毫米）。己酮可可碱还抑制了构成植入物特异性蛋白质组ENDO - 2的一部分蛋白质的生成，而脂多糖诱导产生了另外两种ENDO - 2蛋白质。
免疫调节药物可以调节大鼠子宫内膜异位症植入物的生长以及植入物特异性蛋白质的生成。
