Moran C S, Campbell J H, Simmons D L, Campbell G R
Department of Anatomical Sciences, University of Queensland, Brisbane, Australia.
Arterioscler Thromb. 1994 Aug;14(8):1356-63. doi: 10.1161/01.atv.14.8.1356.
The effect of human leukemia inhibitory factor (hLIF) on the development of atherosclerosis was investigated in an experimental animal model. Two conditions were examined: one in which lesions could arise because of the influence of both "injury" (cuffed vessel) and diet and one in which only the effect of diet could be significant in other areas of the vasculature (aorta). At time zero, the right carotid artery of rabbits (n = 32) was ensheathed in a soft Silastic cuff, and an osmotic minipump (2-mL capacity; 2.5 microL/h; 28 days) containing either hLIF or saline was inserted into the peritoneal cavity. Rabbits were divided into four groups (n = 8): group 1 received normal diet/saline; group 2, normal diet/LIF (30 micrograms.kg-1.d-1); group 3, 1% cholesterol diet/saline; and group 4, 1% cholesterol diet/LIF (30 micrograms.kg-1.d-1). After 28 days, the cholesterol diet (group 3) resulted in a sixfold increase in plasma cholesterol level compared with group 1 rabbits on a normal diet (3.80 +/- 0.50 versus 0.55 +/- 0.01 mmol/L). This was significantly lower (P = .01) with hLIF treatment in group 4 rabbits (2.80 +/- 0.44 mmol/L). Group 2 rabbits had higher aortic tissue cholesterol levels (1.40 +/- 0.35 mg/g) compared with group 1 rabbits on a normal diet (0.10 +/- 0.06 mg/g) (P = .01), whereas hLIF treatment decreased tissue cholesterol levels by 60% in group 4 rabbits (0.60 +/- 0.05 mg/g) (P = .01). Group 3 rabbits developed lipid-filled lesions covering 63.25 +/- 17.66% of the thoracic aorta surface, whereas lesions were significantly reduced (9.88 +/- 8.79%) (P = .01) with LIF treatment (group 4).(ABSTRACT TRUNCATED AT 250 WORDS)
在实验动物模型中研究了人白血病抑制因子（hLIF）对动脉粥样硬化发展的影响。研究了两种情况：一种是由于“损伤”（带套血管）和饮食的影响可能出现病变，另一种是在血管系统的其他部位（主动脉）只有饮食的影响可能显著。在时间零点，将家兔（n = 32）的右颈动脉套上柔软的硅橡胶套，并将含有hLIF或生理盐水的渗透微型泵（容量2 mL；2.5 μL/h；28天）插入腹腔。家兔分为四组（n = 8）：第1组接受正常饮食/生理盐水；第2组，正常饮食/LIF（30 μg·kg⁻¹·d⁻¹）；第3组，1%胆固醇饮食/生理盐水；第4组，1%胆固醇饮食/LIF（30 μg·kg⁻¹·d⁻¹）。28天后，与正常饮食的第1组家兔相比，胆固醇饮食（第3组）使血浆胆固醇水平增加了六倍（3.80±0.50对0.55±0.01 mmol/L）。第4组家兔接受hLIF治疗后，该水平显著降低（P = 0.01）（2.80±0.44 mmol/L）。与正常饮食的第1组家兔相比，第2组家兔的主动脉组织胆固醇水平更高（1.40±0.35 mg/g对0.10±0.06 mg/g）（P = 0.01），而hLIF治疗使第4组家兔的组织胆固醇水平降低了60%（0.60±0.05 mg/g）（P = 0.01）。第3组家兔出现脂质填充病变，覆盖胸主动脉表面的63.25±17.66%，而LIF治疗（第4组）使病变显著减少（9.88±8.79%）（P = 0.01）。（摘要截断于250字）
