Muller W A, Klotz L C
Biochim Biophys Acta. 1975 Jan 20;378(2):171-85. doi: 10.1016/0005-2787(75)90105-7.
We have found that high concentrations of EDTA (greater than 0.024 M) are necessary to produce large, constant numbers of intact replicating Bacillus subtilis chromosomes in lysates of log phase cells. The retardation time of replicating chromosomes in log phase cell lysates is about double that for chromosomes in stationary phase cell lysates, thus making measurement of retardation time a sensitive way to detect and study replicating chromosomes. A theory is developed to predict retardation times for many possible models of DNA replication. The retardation time data on log phase cells is sufficient to eliminate many replication models, but many possibilities remain.
我们发现，要在对数期细胞裂解物中产生大量恒定数量的完整复制型枯草芽孢杆菌染色体，需要高浓度的乙二胺四乙酸（EDTA，大于0.024 M）。对数期细胞裂解物中复制染色体的延迟时间约为稳定期细胞裂解物中染色体延迟时间的两倍，因此延迟时间的测量成为检测和研究复制染色体的一种灵敏方法。我们提出了一个理论，用于预测许多可能的DNA复制模型的延迟时间。对数期细胞的延迟时间数据足以排除许多复制模型，但仍有多种可能性存在。
