枯草芽孢杆菌中的细胞壁合成与脱氧核糖核酸复制的起始
Cell wall synthesis and initiation of deoxyribonucleic acid replication in Bacillus subtilis.
作者信息
Sandler N, Keynan A
出版信息
J Bacteriol. 1981 Nov;148(2):443-9. doi: 10.1128/jb.148.2.443-449.1981.
We have observed a connection between cell wall synthesis and the initiation of chromosome replication in Bacillus subtilis. Initiation of chromosome replication was prevented in synchronous cultures in the presence of the cell wall synthesis inhibitor vancomycin. When vancomycin was added to the cultures after initiation of chromosome replication, one round of replication was completed but no reinitiation occurred. Similar results were obtained when cell wall synthesis was inhibited by ristocetin, cycloserine, cloxacillin, or cephaloridine. When sucrose was added to the medium, initiation of deoxyribonucleic acid replication occurred in the presence of vancomycin, to an extent which allowed replication of no more than approximately one-half of the deoxyribonucleic acid of the culture. The same was found in cultures of spheroplasts of B. subtilis. However, initiation of chromosome replication in spheroplasts was completely insensitive to cloxacillin.
我们已经观察到枯草芽孢杆菌细胞壁合成与染色体复制起始之间的联系。在细胞壁合成抑制剂万古霉素存在的情况下，同步培养物中染色体复制的起始受到抑制。当在染色体复制起始后向培养物中添加万古霉素时，一轮复制完成，但没有再次起始。当用瑞斯托菌素、环丝氨酸、氯唑西林或头孢菌素抑制细胞壁合成时，也得到了类似的结果。当向培养基中添加蔗糖时，在万古霉素存在的情况下发生了脱氧核糖核酸复制的起始，其程度使得培养物中不超过约一半的脱氧核糖核酸得以复制。在枯草芽孢杆菌原生质体培养物中也发现了同样的情况。然而，原生质体中染色体复制的起始对氯唑西林完全不敏感。
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