Steindel M, Dias Neto E, Pinto C J, Grisard E C, Menezes C L, Murta S M, Simpson A J, Romanha A J
Departmento de Microbiologia e Parasitologia Universidade Federal de Santa Catarina C.P. Brazil.
J Eukaryot Microbiol. 1994 May-Jun;41(3):261-7. doi: 10.1111/j.1550-7408.1994.tb01506.x.
Sixteen Trypanosoma rangeli strains were compared by isoenzyme and randomly amplified polymorphic DNA (RAPD) analysis. Eight strains were isolated from either Rhodnius prolixus or Homo sapiens from Honduras, Colombia and Venezuela. Another eight strains were isolated from either Panstrongylus megistus or the rodent Echimys dasythrix from the State of Santa Catarina, southern Brazil. All six T. rangeli strains isolated from P. megistus were co-infections with Trypanosoma cruzi, demonstrating an overlap of the sylvatic cycles of these parasites and that the accurate identification of species is of utmost importance. Both isoenzyme and RAPD analysis revealed two distinct groups of T. rangeli strains, one formed by the strains from Santa Catarina and the other, by the strains from Honduras, Colombia and Venezuela. With the five enzymes used, all the strains from Santa Catarina had identical profiles which overlapped with those of the other regions only in the pattern obtained with malic enzyme. Analysis of 138 RAPD bands by means of an unweighted pair group method analysis (UPGMA) phenogram using the Dice similarity coefficient allowed the separation of the two groups based on their divergence at a lower level of similarity than the phenon line. We show that the identification of T. cruzi and T. rangeli in naturally mixed infections is readily achieved by either RAPD or isoenzyme analysis.
通过同工酶和随机扩增多态性DNA（RAPD）分析对16株克氏锥虫进行了比较。其中8株分别从洪都拉斯、哥伦比亚和委内瑞拉的长红猎蝽或人类中分离得到。另外8株则从巴西南部圣卡塔琳娜州的大锥蝽或啮齿动物多毛棘鼠中分离得到。从大锥蝽中分离出的所有6株克氏锥虫均与克氏锥虫共感染，这表明这些寄生虫的野生传播周期存在重叠，准确鉴定物种至关重要。同工酶和RAPD分析均显示克氏锥虫菌株分为两个不同的组，一组由来自圣卡塔琳娜州的菌株组成，另一组由来自洪都拉斯、哥伦比亚和委内瑞拉的菌株组成。在所使用的5种酶中，所有来自圣卡塔琳娜州的菌株具有相同的图谱，仅在苹果酸酶所得图谱上与其他地区的图谱有重叠。使用Dice相似系数，通过非加权配对组方法分析（UPGMA）系统树图对138条RAPD条带进行分析，基于两组在低于类群线的相似性水平上的差异，实现了两组的分离。我们表明，通过RAPD或同工酶分析可以很容易地在自然混合感染中鉴定克氏锥虫和克氏锥虫。
