天然叶酸化合物的研究。IV. 大豆和牛奶中的叶酸化合物。
Studies of folic acid compounds in nature. IV. Folic acid compounds in soybeans and cow milk.
作者信息
Shin Y S, Kim E S, Watson J E, Stokstad E L
出版信息
Can J Biochem. 1975 Mar;53(3):338-43. doi: 10.1139/o75-047.
Folate compounds in soybean and cow's mild were identified by Sephadex chromatography and differential microbiological assaymsoybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-CHO-H4PteGlun(1-6) constituted 65-70% of total folate activity; Cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). In contrast to soybean, 90-95% of milk folate was in the 5-CH3h4pteGlun(1-7) form.
通过葡聚糖凝胶色谱法和微生物差异分析法鉴定了大豆和牛奶中的叶酸化合物。大豆主要含有单谷氨酸盐（约52%）、一些双谷氨酸盐（约16%）和多谷氨酸盐（以五谷氨酸盐为主）。5-CHO-H4PteGlun(1-6)占总叶酸活性的65-70%；牛奶含有单谷氨酸盐（60%）和多谷氨酸盐（从二聚体到七聚体共轭物）。与大豆不同，牛奶中90-95%的叶酸为5-CH3h4pteGlun(1-7)形式。
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