长叶生菜中的叶酸化合物。

Folic acid compounds in romaine lettuce.

作者信息

Batra K K, Wagner J R, Stokstad E L

出版信息

Can J Biochem. 1977 Aug;55(8):865-8. doi: 10.1139/o77-127.

Abstract

The composition of folate coenzymes in romaine lettuce was studied. Lettuce extract was purified on QAE-Sephadex A-25 and folate compounds were separated into a monoglutamate fraction and a polyglutamate fraction by chromatography on Sephadex G-15. Both the mono- and poly-glutamate fractions were resolved on DEAE-cellulose. Positive identification of DEAE peaks was made by further cochromatography with high specific activity radioactive marker folate compounds and with differential microbiological assay. The distribution of folate compounds in lettuce is as follows: 32% 5-CH3-H4PteGlu; 1% 5-CHO-H4PteGlu; 3% 5-CHO-H4PteGlu4; 9% 5-CH3-H4PteGlu4; 13% 5-CHO-H4PteGlu5; and 31% 5-CH3-H4PteGlu5.

摘要

对长叶生菜中叶酸辅酶的组成进行了研究。生菜提取物在QAE-葡聚糖凝胶A-25上进行纯化，叶酸化合物通过在葡聚糖凝胶G-15上的色谱法分离为单谷氨酸部分和多谷氨酸部分。单谷氨酸和多谷氨酸部分都在二乙氨基乙基纤维素上进行分离。通过与高比活性放射性标记叶酸化合物进一步共色谱分析和差异微生物测定，对二乙氨基乙基纤维素峰进行了阳性鉴定。生菜中叶酸化合物的分布如下：32%为5-甲基-四氢叶酸单谷氨酸；1%为5-甲酰基-四氢叶酸；3%为5-甲酰基-四氢叶酸四谷氨酸；9%为5-甲基-四氢叶酸四谷氨酸；13%为5-甲酰基-四氢叶酸五谷氨酸；31%为5-甲基-四氢叶酸五谷氨酸。