Rebers P A, Heddleston K L, Wright B, Gillette K
Carbohydr Res. 1975 Mar;40(1):99-110. doi: 10.1016/s0008-6215(00)82672-5.
Turkey antisera induced with formolized Pasteurella multocida-infected tissues (T antisera) passively cross-immunized 48 of 55 chickens against a challenge dose of P. multocida organisms, from which 0 of 15 controls survived. However, turkey antisera induced with formalin-killed, agar-cultured P. multocida cells (A antisera) passively cross-immunized only 4 of 30 chickens. Cross-immunity refers to protection against a different immunologic type of P. multocida. Quantitative precipitin reactions of the A and T antisera with antigens from agar-cultured cells showed that more antibody was present in the A than in the T antisera. However, antigens extracted from the infected tissues reacted with the T and not with the A antisera in the Ouchterlony procedure, demonstrating qualitative differences between the agar-cultured antigens and those extracted from the infected tissue. The gel precipitins isolated from the A and T antisera were characterized as 7S immunoglobulins, which behaved in immunoelectrophoresis as would be expected for a IgG immunoglobulin. The IgG fraction from the T antiserum passively cross-immunized chickens almost as well as the whole antiserum; hence, the IgG antibody is a major factor in cross-immunity.
用甲醛处理的多杀性巴氏杆菌感染组织诱导产生的火鸡抗血清（T抗血清），对55只鸡中的48只进行了被动交叉免疫，使其能抵抗多杀性巴氏杆菌的攻击剂量，而15只对照鸡中无一存活。然而，用福尔马林灭活的琼脂培养多杀性巴氏杆菌细胞诱导产生的火鸡抗血清（A抗血清），仅对30只鸡中的4只进行了被动交叉免疫。交叉免疫是指对不同免疫类型的多杀性巴氏杆菌的保护作用。A抗血清和T抗血清与琼脂培养细胞抗原的定量沉淀反应表明，A抗血清中的抗体比T抗血清中的多。然而，在双向琼脂扩散试验中，从感染组织中提取的抗原与T抗血清发生反应，而不与A抗血清反应，这表明琼脂培养抗原与从感染组织中提取的抗原存在质的差异。从A抗血清和T抗血清中分离出的凝胶沉淀素被鉴定为7S免疫球蛋白，在免疫电泳中表现为IgG免疫球蛋白所预期的行为。T抗血清的IgG组分对鸡的被动交叉免疫效果几乎与整个抗血清相同；因此，IgG抗体是交叉免疫中的主要因素。
