Rodriguez-Arnaiz R, Aranda J H
Laboratorio de Genética, Facultad de Ciencias, UNAM, Coyoacán, D.F.
Environ Mol Mutagen. 1994;24(1):75-9. doi: 10.1002/em.2850240110.
Aromatic amines represent a category of classical environmental hazards which require biotransformation in order to exert their mutagenic and carcinogenic effects. The mutagenic activity of several aromatic amines was tested by means of the w/w+ somatic assay of Drosophila melanogaster employing a wild-type strain Leiden Standard (LS) and an insecticide-resistant stock Hikone-R (HK-R). Four monocyclicortho-anisidine (O-AN), ortho-toluidine (O-TO), 2,4-diaminoanisole (2,4-DAA) and 4-nitro-o-phenylenediamine (4-N-o-PDA), and one bicyclic- 4,4'-oxidianiline (4,4'-ODA) aromatic amines were tested by chronic exposure. Two separate experiments were run, and concurrent controls were treated with the solvent mixture. Results show that the ortho-anilines (O-AN and O-TO) were active in the assay, while the diamines containing a methoxy (2,4-DAA) or a nitro group (4-N-o-PDA) were inactive. The bicyclic aromatic amine, 4,4'-ODA, was the most potent. The genotoxic responsiveness of both strains was very similar.
芳香胺是一类典型的环境危害物，需要通过生物转化才能发挥其诱变和致癌作用。采用野生型莱顿标准品系（LS）和抗杀虫剂品系姬路-R（HK-R），通过黑腹果蝇的w/w+体细胞试验，对几种芳香胺的诱变活性进行了测试。通过长期暴露试验，对四种单环邻茴香胺（O-AN）、邻甲苯胺（O-TO）、2,4-二氨基苯甲醚（2,4-DAA）和4-硝基邻苯二胺（4-N-o-PDA）以及一种双环-4,4'-氧化二苯胺（4,4'-ODA）芳香胺进行了测试。进行了两个独立的实验，并用溶剂混合物处理了同步对照。结果表明，邻苯胺（O-AN和O-TO）在试验中具有活性，而含有甲氧基（2,4-DAA）或硝基（4-N-o-PDA）的二胺则无活性。双环芳香胺4,4'-ODA的活性最强。两个品系的遗传毒性反应非常相似。
