Burchell B, Leakey J, Dutton G J
Enzyme. 1975;20(3):156-64. doi: 10.1159/000458934.
Activity of UDP-glucuronyltransferase (GT) towards o-aminophenol, p-nitrophenol and bilirubin has been followed in portions of mouse liver stored for periods up to 24 h and in mouse-liver homogenates stored under similar conditions of time and temperature. In both preparations and for all three substrates the pattern of change of GT activity was closely similar. Activity decreased initially, then rose to an optimum higher than in fresh tissue before finally falling. Overall glucuronidation, as measured in slices, also follwed this pattern. The effect of cycloheximide and of detergents, and the levels of UDP-glucose dehydrogenase, UDP-glucuronic acid and aniline hydroxylase were also studied. It is concluded that autolysing liver tissue passes through a period of increased GT activity and glucuronidation corresponding in onset with spontaneous activation of GT in stored homogenates and probably originating by a similiar mechanism. This increase contrasts with progressive fall in hydroxylating activity. The latency of GT in vivo and the value of its activation in damaged liver are discussed.
已对储存长达24小时的部分小鼠肝脏以及在类似时间和温度条件下储存的小鼠肝脏匀浆中，尿苷二磷酸葡萄糖醛酸基转移酶（GT）对邻氨基酚、对硝基苯酚和胆红素的活性进行了追踪研究。在这两种制剂中，对于所有三种底物，GT活性的变化模式都非常相似。活性最初下降，然后上升至高于新鲜组织中的最佳水平，最终又下降。在切片中测量的总体葡萄糖醛酸化也遵循这种模式。还研究了放线菌酮和去污剂的作用，以及尿苷二磷酸葡萄糖脱氢酶、尿苷二磷酸葡萄糖醛酸和苯胺羟化酶的水平。得出的结论是，自溶的肝脏组织会经历一段GT活性和葡萄糖醛酸化增加的时期，其开始时间与储存匀浆中GT的自发激活相对应，并且可能起源于类似的机制。这种增加与羟化活性的逐渐下降形成对比。讨论了GT在体内的潜伏性及其在受损肝脏中的激活价值。
