Lenler-Petersen P, Grove A, Brock A, Jelnes R
Dept of Thoracic Surgery, Aalborg Hospital, Denmark.
Eur Respir J. 1994 May;7(5):941-5.
Biochemical analysis and immunohistochemical techniques support the theory that hyperamylasaemia in lung cancer is due to amylase production in carcinoma cells. The vast majority of amylase-producing carcinomas are adenocarcinomas with amylase isoenzyme similar to the salivary type. This prospective study assesses alpha-amylase expression in resectable lung cancer. Seventy four patients with resectable lung cancer were studied. Amylase activity in tumour tissue was analysed and isoamylase identification performed. Immunohistochemical analysis was performed using a polyclonal rabbit antibody against human salivary amylase. Hyperamylasaemia occurred in 13 out of 70 patients. Increased amylase activity in tumour tissue was found in 10 out of 52 cases, of which only two were associated with hyperamylasaemia. With the exception of one large cell carcinoma and one squamous cell carcinoma, the tumours were adenocarcinomas. Immunohistochemical analysis revealed amylase expression in seven adenocarcinomas and two adenosquamous carcinomas. In conclusion, immunohistochemical amylase expression was restricted to carcinomas with adenomatous differentiation. Biochemical analysis confirmed amylase production in 5 of 7 cases examined, the tissue amylase isoenzymes being of salivary type. However, hyperamylasaemia and a slightly increased amylase activity in tumour tissue may be caused by factors other than amylase-producing carcinoma cells.
生化分析和免疫组化技术支持这样一种理论，即肺癌中的高淀粉酶血症是由于癌细胞产生淀粉酶所致。绝大多数产生淀粉酶的癌是腺癌，其淀粉酶同工酶与唾液型相似。这项前瞻性研究评估了可切除肺癌中α-淀粉酶的表达。对74例可切除肺癌患者进行了研究。分析了肿瘤组织中的淀粉酶活性并进行了异淀粉酶鉴定。使用抗人唾液淀粉酶的多克隆兔抗体进行免疫组化分析。70例患者中有13例出现高淀粉酶血症。52例中有10例肿瘤组织中的淀粉酶活性增加，其中只有2例与高淀粉酶血症有关。除1例大细胞癌和1例鳞状细胞癌外，其余肿瘤均为腺癌。免疫组化分析显示7例腺癌和2例腺鳞癌中有淀粉酶表达。总之，免疫组化淀粉酶表达仅限于具有腺瘤样分化的癌。生化分析证实，在所检查的7例中有5例产生淀粉酶，组织淀粉酶同工酶为唾液型。然而，高淀粉酶血症和肿瘤组织中淀粉酶活性略有增加可能是由产生淀粉酶的癌细胞以外的因素引起的。
