Thumm M, Egner R, Koch B, Schlumpberger M, Straub M, Veenhuis M, Wolf D H
Institut für Biochemie, Universität Stuttgart, Germany.
FEBS Lett. 1994 Aug 1;349(2):275-80. doi: 10.1016/0014-5793(94)00672-5.
Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.
液泡（溶酶体）中的蛋白质降解是细胞调控中的一个重要事件。在酵母中，如同在哺乳动物细胞中一样，已发现蛋白质被摄取进入液泡（溶酶体）进行降解的一条主要途径是自噬作用。在酵母中发现这一过程使得能够通过遗传和分子生物学研究阐明其机制。在此，我们报告使用一种快速菌落筛选程序分离出自噬作用缺陷型酵母突变体（自噬突变体）。
