百日咳博德特氏菌bvgS基因中的突变可导致大肠杆菌中fhaB基因表达的改变。
Mutations in the Bordetella pertussis bvgS gene that confer altered expression of the fhaB gene in Escherichia coli.
作者信息
Goyard S, Bellalou J, Mireau H, Ullmann A
机构信息
Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.
出版信息
J Bacteriol. 1994 Aug;176(16):5163-6. doi: 10.1128/jb.176.16.5163-5166.1994.
Abstract
The bvg locus of Bordetella pertussis, required for coordinate regulation of virulence genes in response to environmental signals, encodes two proteins, BvgS and BvgA, that belong to the bacterial two-component signal transduction systems. We have isolated spontaneous mutations of the bvg locus in Escherichia coli and analyzed their effects on the expression of fhaB::lacZY transcriptional fusions. The mutations, localized in the linker and transmitter domain of BvgS, result in increased activation of fhaB and/or in insensitivity to a modulating agent, nicotinic acid.
摘要
百日咳博德特氏菌的bvg位点负责响应环境信号对毒力基因进行协同调控，它编码两种属于细菌双组分信号转导系统的蛋白质，即BvgS和BvgA。我们在大肠杆菌中分离出了bvg位点的自发突变，并分析了它们对fhaB::lacZY转录融合表达的影响。这些突变位于BvgS的连接区和传递结构域，导致fhaB的激活增加和/或对调节剂烟酸不敏感。
相似文献
1
Mutations in the Bordetella pertussis bvgS gene that confer altered expression of the fhaB gene in Escherichia coli.
J Bacteriol. 1994 Aug;176(16):5163-6. doi: 10.1128/jb.176.16.5163-5166.1994.
2
Mutations which result in constitutive expression of the Bordetella pertussis filamentous haemagglutinin gene.
Res Microbiol. 1995 Jun;146(5):363-70. doi: 10.1016/0923-2508(96)80282-8.
3
Constitutive sensory transduction mutations in the Bordetella pertussis bvgS gene.
J Bacteriol. 1992 Feb;174(3):970-9. doi: 10.1128/jb.174.3.970-979.1992.
4
BvgAS is sufficient for activation of the Bordetella pertussis ptx locus in Escherichia coli.
J Bacteriol. 1995 Nov;177(22):6477-85. doi: 10.1128/jb.177.22.6477-6485.1995.
5
The bvgA gene of Bordetella pertussis encodes a transcriptional activator required for coordinate regulation of several virulence genes.
J Bacteriol. 1989 Nov;171(11):6338-44. doi: 10.1128/jb.171.11.6338-6344.1989.
6
Autophosphorylation and phosphotransfer in the Bordetella pertussis BvgAS signal transduction cascade.
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1163-7. doi: 10.1073/pnas.91.3.1163.
7
Mutations in the linker region of BvgS abolish response to environmental signals for the regulation of the virulence factors in Bordetella pertussis.
Gene. 1994 Dec 2;150(1):123-7. doi: 10.1016/0378-1119(94)90870-2.
8
Structural and genetic analysis of the bvg locus in Bordetella species.
Mol Microbiol. 1991 Oct;5(10):2481-91. doi: 10.1111/j.1365-2958.1991.tb02093.x.
9
Analysis of Bordetella pertussis virulence gene regulation by use of transcriptional fusions in Escherichia coli.
J Bacteriol. 1989 Nov;171(11):6345-8. doi: 10.1128/jb.171.11.6345-6348.1989.
10
The BvgAS Regulon of .
mBio. 2017 Oct 10;8(5):e01526-17. doi: 10.1128/mBio.01526-17.
引用本文的文献
1
Structural insight into the role of the PAS domainfor signal transduction in sensor-kinase BvgS.
J Bacteriol. 2021 May 1;203(9). doi: 10.1128/JB.00614-20. Epub 2021 Feb 22.
2
Conformational Changes of an Interdomain Linker Mediate Mechanical Signal Transmission in Sensor Kinase BvgS.
J Bacteriol. 2017 Aug 22;199(18). doi: 10.1128/JB.00114-17. Print 2017 Sep 15.
3
Genome Structural Diversity among 31 Bordetella pertussis Isolates from Two Recent U.S. Whooping Cough Statewide Epidemics.
mSphere. 2016 May 11;1(3). doi: 10.1128/mSphere.00036-16. eCollection 2016 May-Jun.
4
Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli.
Mol Microbiol. 2014 Sep;93(5):911-27. doi: 10.1111/mmi.12704. Epub 2014 Jul 24.
5
In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.
Mol Microbiol. 2013 Apr;88(1):156-72. doi: 10.1111/mmi.12177. Epub 2013 Mar 14.
6
Conserved sequence motifs in the unorthodox BvgS two-component sensor protein of Bordetella pertussis.
Mol Gen Genet. 1996 Aug 27;252(1-2):169-76. doi: 10.1007/BF02173217.
7
Integration of multiple domains in a two-component sensor protein: the Bordetella pertussis BvgAS phosphorelay.
EMBO J. 1996 Mar 1;15(5):1028-36.
8
BvgAS is sufficient for activation of the Bordetella pertussis ptx locus in Escherichia coli.
J Bacteriol. 1995 Nov;177(22):6477-85. doi: 10.1128/jb.177.22.6477-6485.1995.
本文引用的文献
1
Antigenic modulation of Bordetella pertussis.
J Hyg (Lond). 1960 Mar;58(1):57-93. doi: 10.1017/s0022172400038134.
2
Environmental regulation of virulence factors in Bordetella species.
Bioessays. 1993 Feb;15(2):99-104. doi: 10.1002/bies.950150205.
3
Functional analysis of the cya promoter of Bordetella pertussis.
Mol Microbiol. 1993 Mar;7(5):693-704. doi: 10.1111/j.1365-2958.1993.tb01160.x.
4
Autophosphorylation and phosphotransfer in the Bordetella pertussis BvgAS signal transduction cascade.
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1163-7. doi: 10.1073/pnas.91.3.1163.
5
The essential tension: opposed reactions in bacterial two-component regulatory systems.
Trends Microbiol. 1993 Nov;1(8):306-10. doi: 10.1016/0966-842x(93)90007-e.
6
DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene.
J Mol Biol. 1986 Jul 5;190(1):113-7. doi: 10.1016/0022-2836(86)90080-x.
7
Positive regulation of pertussis toxin expression.
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3913-7. doi: 10.1073/pnas.85.11.3913.
8
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Nucleic Acids Res. 1988 Feb 25;16(4):1563-75. doi: 10.1093/nar/16.4.1563.
9
Protein phosphorylation and regulation of adaptive responses in bacteria.
Microbiol Rev. 1989 Dec;53(4):450-90. doi: 10.1128/mr.53.4.450-490.1989.
10
Analysis of Bordetella pertussis virulence gene regulation by use of transcriptional fusions in Escherichia coli.
J Bacteriol. 1989 Nov;171(11):6345-8. doi: 10.1128/jb.171.11.6345-6348.1989.
Zotero 插件