通过定量聚合酶链反应测定慢性丙型肝炎患者血清丙型肝炎病毒核糖核酸水平；与血清谷草转氨酶的相关性

Serum HCV RNA levels in chronic HCV hepatitis measured by quantitative PCR assay; correlation with serum AST.

作者信息

Kumar U, Thomas H C, Monjardino J

机构信息

Department of Medicine, St. Mary's Hospital Medical School, Imperial College of Science, Technology, and Medicine, London, UK.

出版信息

J Virol Methods. 1994 Apr;47(1-2):95-102. doi: 10.1016/0166-0934(94)90069-8.

DOI:10.1016/0166-0934(94)90069-8

PMID:8051236

Abstract

A quantitative PCR assay is described for serum HCV RNA which is based on a recombinant competitive titrating RNA template. This template is derived from the 5' non-coding region of the genome and generates a shorter PCR product (93 bp) than that from the serum-derived wild type genomes (279) bp from the same sets of primers. By using this assay we have found serum HCV RNA concentrations ranging between 3.3 x 10(2) and 6.6 x 10(8) viral genomes/ml of serum in 15 samples from 10 different patients, 9 cases of chronic hepatitis and one case of fulminant hepatitis. Comparison of viraemias with serum transaminase levels has shown a good correlation for individual patients between these two parameters during chronic HCV hepatitis.

摘要

描述了一种基于重组竞争性滴定RNA模板的血清丙型肝炎病毒（HCV）RNA定量聚合酶链反应（PCR）检测方法。该模板源自基因组的5'非编码区，与同一组引物扩增血清来源的野生型基因组（279 bp）相比，产生的PCR产物较短（93 bp）。通过使用该检测方法，我们在来自10名不同患者的15份样本（9例慢性肝炎和1例暴发性肝炎）中发现血清HCV RNA浓度在3.3×10²至6.6×10⁸病毒基因组/毫升血清之间。在慢性HCV肝炎期间，个体患者的病毒血症与血清转氨酶水平的比较显示这两个参数之间具有良好的相关性。