Watanabe T
Department of Oral Bacteriology, Hokkaido University School of Dentistry, Japan.
J Clin Microbiol. 1994 May;32(5):1343-5. doi: 10.1128/jcm.32.5.1343-1345.1994.
The growth of Mycoplasma salivarium ATCC 23064 and Mycoplasma orale ATCC 15539 was inhibited by MnCl2. The growth-inhibitory effect was much more remarkable on M. orale than M. salivarium and was much more remarkable in medium supplemented with 10% (vol/vol) horse serum (HS) than 20% (vol/vol) HS. It was suggested that isolates of Mycoplasma from the oral cavity could be roughly identified as either M. salivarium and M. orale by examination of the growth (color changes) in PPLO broth supplemented with 10% (vol/vol) HS and 0.2 mM MnCl2.
唾液支原体ATCC 23064和口腔支原体ATCC 15539的生长受到MnCl2的抑制。这种生长抑制作用对口腔支原体的影响比对唾液支原体更为显著,并且在添加10%(体积/体积)马血清(HS)的培养基中比在添加20%(体积/体积)HS的培养基中更为显著。有人提出,通过检测添加10%(体积/体积)HS和0.2 mM MnCl2的PPLO肉汤中的生长情况(颜色变化),可以大致将从口腔分离出的支原体鉴定为唾液支原体或口腔支原体。