Chang I C, Lee T P, Matrone G
J Nutr. 1975 May;105(5):624-30. doi: 10.1093/jn/105.5.624.
Colostrum-free newborn pigs were raised artificially to investigate the appearance of ceruloplasmin in the serum of piglets. The sensitivity of the p-phenylenediamine oxidase activity assay, commonly used as a measure of ceruloplasmin, has been increased 10-fold. Based on this activity, no evidence was found that this copper protein was present in the serum of baby pigs at birth, although some evidence was obtained that the protein moiety (apoceruloplasmin) was present. Holoceruloplasmin usually became detectable in the serum 10-15 hours after birth. Its concentration increased slowly at first and then at an accelerated rate. The ceruloplasmin in the sera of 2-3-day-old piglets was isolated and compared with that isolated from the serum of adults. Contrary to a previous report, the physical and chemical properties of ceruloplasmin synthesized during the first 3 days of life of the piglet showed no significant difference from that synthesized by the adult.
使用无初乳的新生仔猪进行人工饲养，以研究仔猪血清中铜蓝蛋白的出现情况。常用于测量铜蓝蛋白的对苯二胺氧化酶活性测定法的灵敏度提高了10倍。基于该活性，未发现出生时仔猪血清中存在这种铜蛋白的证据，尽管获得了一些证据表明存在蛋白质部分（脱辅基铜蓝蛋白）。全铜蓝蛋白通常在出生后10 - 15小时在血清中可检测到。其浓度起初缓慢增加，然后加速上升。分离出2 - 3日龄仔猪血清中的铜蓝蛋白，并与从成年猪血清中分离出的铜蓝蛋白进行比较。与之前的一份报告相反，仔猪出生后头3天合成的铜蓝蛋白的物理和化学性质与成年猪合成的铜蓝蛋白相比无显著差异。
