Chang I C, Milholland D C, Matrone G
J Nutr. 1976 Sep;106(9):1343-50. doi: 10.1093/jn/106.9.1343.
The sequence of ceruloplasmin development and limiting factors controlling this process in neonatal piglets was studied in a randomized block design. Guided by the change in serum ceruloplasmin levels, the liver copper concentrations of piglets fed copper-deficient and copper-supplemented diets were compared in three different periods. The plasma of piglets was devoid of both apo- and holo-ceruloplasmin at birth. The system responsible for the synthesis of apoceruloplasmin developed shortly after birth as indicated by a steady increase in serum ceruloplasmin activity during the first week without being affected by the diet. Copper apparently was not the limiting factor at this stage since very high concentrations of copper were found in the livers of newborn piglets. However, the liver copper was depleted quickly within 1 to 2 weeks and became the limiting factor if the diet was deficient in copper. The livers of these piglets were fractionated by differential centrifugation to compare the liver copper distribution in the two dietary groups with and without copper supplementation. The treatments did not affect the copper distribution on a percentage of total liver copper basis. However, fractionation study revealed that, unlike adult pig livers, about 70% to 80% of copper was present in the heavier particle fractions of piglet livers.
采用随机区组设计研究了新生仔猪血浆铜蓝蛋白的发育序列以及控制这一过程的限制因素。以血清铜蓝蛋白水平的变化为指导，比较了在三个不同时期，饲喂缺铜和补铜日粮的仔猪肝脏铜浓度。仔猪出生时血浆中既没有脱辅基铜蓝蛋白也没有全铜蓝蛋白。出生后不久，负责合成脱辅基铜蓝蛋白的系统开始发育，这表现为第一周血清铜蓝蛋白活性稳步上升，且不受日粮影响。由于在新生仔猪肝脏中发现了非常高浓度的铜，铜显然在这个阶段不是限制因素。然而，肝脏铜在1至2周内迅速耗尽，如果日粮缺铜，肝脏铜就会成为限制因素。通过差速离心对这些仔猪的肝脏进行分级分离，以比较两个日粮组（补铜组和不补铜组）肝脏铜的分布情况。处理对以肝脏铜总量百分比表示的铜分布没有影响。然而，分级分离研究表明，与成年猪肝不同，仔猪肝脏中约70%至80%的铜存在于较重的颗粒部分。
