Miyashita N, Lijima Y, Matsumoto A
Department of Microbiology, Kawasaki Medical School, Okayama, Japan.
Microbiol Immunol. 1994;38(1):81-5. doi: 10.1111/j.1348-0421.1994.tb01747.x.
The sensitivity and specificity of the polymerase chain reaction (PCR) test kit, AMPLICOR Chlamydia trachomatis, were examined by the use of purified elementary bodies (EBs), cells having inclusions containing reticulate bodies alone and 20 clinical isolates. The numbers of EB and inclusion of C. trachomatis at the detection limit were determined to be approximately 2 to 4 EBs and one inclusion per assay, respectively. No reaction occurred for C. psittaci and C. pneumoniae. All clinical isolates were positively reacted in the PCR assay.
通过使用纯化的原体（EBs）、仅含有包含网状体的包涵体的细胞以及20株临床分离株，对聚合酶链反应（PCR）检测试剂盒AMPLICOR沙眼衣原体的敏感性和特异性进行了检测。在检测限下，沙眼衣原体的EB数量和包涵体数量分别确定为每次检测约2至4个EB和1个包涵体。鹦鹉热衣原体和肺炎衣原体未出现反应。所有临床分离株在PCR检测中均呈阳性反应。
