用于检测沙眼衣原体的连接酶链反应检测试剂盒的敏感性和特异性评估。
Evaluation of the sensitivity and specificity of a ligase chain reaction test kit for the detection of Chlamydia trachomatis.
作者信息
Miyashita N, Matsumoto A, Niki Y, Matsushima T
机构信息
Department of Medicine, Kawasaki Medical School, Okayama, Japan.
出版信息
J Clin Pathol. 1996 Jun;49(6):515-7. doi: 10.1136/jcp.49.6.515.
Abstract
The sensitivity and specificity of a newly developed ligase chain reaction (LCR) test kit were examined by the use of highly purified elementary bodies (EBs) and in situ inclusions containing reticulate bodies only. The performance of the LCR kit was compared with a commercially available polymerase chain reaction (PCR) test kit, AMPLICOR Chlamydia trachomatis. The number of EBs and inclusions of C trachomatis, respectively, at the detection limit of both kits were two EBs and one inclusion per assay. Neither kit cross-reacted with C pneumoniae, C psittaci and C pecorum EBs or reticulate bodies.
摘要
通过使用高度纯化的原体（EBs）和仅含有网状体的原位包涵体，对新开发的连接酶链反应（LCR）检测试剂盒的灵敏度和特异性进行了检测。将LCR试剂盒的性能与市售的聚合酶链反应（PCR）检测试剂盒AMPLICOR沙眼衣原体进行了比较。两种试剂盒的检测限分别为每个检测中两个EBs和一个包涵体。两种试剂盒均未与肺炎衣原体、鹦鹉热衣原体和猪衣原体的EBs或网状体发生交叉反应。
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本文引用的文献
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