Frew R, Hamilton M G, Lundy P M
Pharmacology and Therapeutics, Defense Research Establishment Suffield, Alberta, Canada.
Toxicon. 1994 Apr;32(4):511-5. doi: 10.1016/0041-0101(94)90303-4.
Venom from the funnel-web spider Hololena curta was added to the Krebs-Henseleit solution bathing isolated ring preparations of rat thoracic aorta, suspended in water-jacketed organ baths, for the purpose of tension recordings. Hololena curta venom at dilutions of 1:100,000 to 1:1000 caused a marked vasoconstriction, which was completely inhibited by the alpha 1 adrenoceptor antagonist prazosin (1 microm). The vasoconstriction appears to be due to the direct effects on alpha 1 adrenoceptors of a venom constituent, which we have identified using HPLC/ECD as the catecholamine, noradrenaline.
为了进行张力记录，将漏斗网蜘蛛（Hololena curta）的毒液添加到置于水套式器官浴中、用于浴灌大鼠胸主动脉分离环制剂的克氏-亨氏溶液中。稀释至1:100,000至1:1000的Hololena curta毒液可引起明显的血管收缩，这种收缩可被α1肾上腺素能受体拮抗剂哌唑嗪（1微摩尔）完全抑制。血管收缩似乎是由于毒液中的一种成分直接作用于α1肾上腺素能受体所致，我们已通过高效液相色谱/电化学检测法（HPLC/ECD）鉴定该成分为儿茶酚胺——去甲肾上腺素。
