Suzuki N, Iwatsubo T, Odaka A, Ishibashi Y, Kitada C, Ihara Y
Discovery Research Division, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Am J Pathol. 1994 Aug;145(2):452-60.
We developed two highly sensitive enzyme immunoassays for beta-protein with different specificities. One is specific for beta 1-40, while the other is equally sensitive to beta 1-38, beta 1-39, beta 1-40, and beta 1-42. With the enzyme immunoassays we investigated whether the soluble fraction from brain tissue contains beta 1-40 or other species of beta-protein. Aged control and Alzheimer's diseased brains showed highly variable values of beta 1-40, which was found to be the major beta species in their extracts. High tissue content of soluble beta 1-40 was not correlated to the abundance of senile plaques but was invariably associated with cerebral amyloid angiopathy. Thus, the tissue level of soluble beta 1-40 should be useful for the quantification of cerebral amyloid angiopathy.
我们开发了两种针对β蛋白的具有不同特异性的高灵敏度酶免疫测定法。一种对β1-40具有特异性，而另一种对β1-38、β1-39、β1-40和β1-42同样敏感。通过这些酶免疫测定法，我们研究了脑组织的可溶性部分是否含有β1-40或其他种类的β蛋白。老年对照大脑和阿尔茨海默病大脑显示出β1-40的高度可变值，β1-40被发现是其提取物中的主要β种类。可溶性β1-40的高组织含量与老年斑的丰度无关，但总是与脑淀粉样血管病相关。因此，可溶性β1-40的组织水平应有助于脑淀粉样血管病的定量分析。
