Boon M E, Kok L P
Leiden Cytology and Pathology Laboratory, The Netherlands.
Micron. 1994;25(2):151-70. doi: 10.1016/0968-4328(94)90040-x.
Microwaves are now widely used in immunohistochemistry for fixing and stabilizing tissue prior to embedding and cutting, for antigen retrieval and for immunoincubations. These techniques can be used for frozen sections and for material embedded in paraffin and plastic. Material prepared in this way shows high contrast in light microscopy. In principle, these microwave methods can also be used for electron microscopy. To be successful in the application of these techniques, insight into the physics of exposure to microwaves and the effects of microwaves on the material is a must. Microwave immunohistochemistry depends on optimal temperature control. To guarantee this, special measures should be taken and dedicated laboratory ovens should be used. The recently developed Coverplate units facilitate immunoincubations in the microwave oven. We show that the total microwave approach, combining microwave fixation, embedding and immunoincubations, is very useful for confocal microscopy.
目前，微波在免疫组织化学中被广泛应用，用于在包埋和切片前固定和稳定组织、进行抗原修复以及免疫孵育。这些技术可用于冰冻切片以及石蜡和塑料包埋的材料。以这种方式制备的材料在光学显微镜下显示出高对比度。原则上，这些微波方法也可用于电子显微镜检查。要成功应用这些技术，必须深入了解微波照射的物理原理以及微波对材料的影响。微波免疫组织化学依赖于最佳温度控制。为确保这一点，应采取特殊措施并使用专用实验室烤箱。最近开发的盖板装置便于在微波炉中进行免疫孵育。我们表明，将微波固定、包埋和免疫孵育相结合的全微波方法对共聚焦显微镜检查非常有用。
