Srinivasan Mythily, Sedmak Daniel, Jewell Scott
Department of Pathology, Tzagournis Medical Research Facility, The Ohio State University, 420 West 12th Avenue, Columbus, OH 43210, USA.
Am J Pathol. 2002 Dec;161(6):1961-71. doi: 10.1016/S0002-9440(10)64472-0.
Clinical and molecular medicines are undergoing a revolution based on the accelerated advances in biotechnology such as DNA microarrays and proteomics. Answers to fundamental questions such as how does the DNA sequence differ between individuals and what makes one individual more prone for a certain disease are eagerly being sought in this postgenomic era. Several government and nonprofit organizations provide the researchers access to human tissues for molecular studies. The tissues procured by the different organizations may differ with respect to fixation and processing parameters that may affect significantly the molecular profile of the tissues. It is imperative that a prospective investigator be aware of the potential contributing factors before designing a project. The purpose of this review is to provide an overview of the methods of human tissue acquisition, fixation, and preservation. In addition, the parameters of procurement and fixation that affect the quality of the tissues at the molecular level are discussed.
基于DNA微阵列和蛋白质组学等生物技术的加速发展，临床和分子医学正在经历一场变革。在这个后基因组时代，人们急切地寻求诸如个体之间DNA序列如何不同以及是什么使一个人更容易患某种疾病等基本问题的答案。一些政府和非营利组织为研究人员提供用于分子研究的人体组织。不同组织获取的组织在固定和处理参数方面可能存在差异，而这些参数可能会显著影响组织的分子特征。前瞻性研究人员在设计项目之前必须了解潜在的影响因素。本综述的目的是概述人体组织获取、固定和保存的方法。此外，还讨论了在分子水平上影响组织质量的获取和固定参数。
