Kok K, van den Berg A, Buchhagen D L, Carritt B, Buys C H
Department of Medical Genetics, University of Groningen, The Netherlands.
Eur J Hum Genet. 1993;1(2):156-63. doi: 10.1159/000472402.
We have developed a general PCR-based method to quantify the amount of a specific mRNA present in a given cell line or tissue. We applied this quantitative PCR to analyse the expression of D8, a human gene which we recently identified in the chromosomal region 3p21, the common deletion region of lung cancer. Our PCR-aided assay shows that in most lung-cancer-derived cell lines the amount of D8 transcripts is only 2% or less of that in normal lung tissue. The virtual absence of expression may imply some role of the gene in the development of lung cancer.
我们开发了一种基于聚合酶链反应（PCR）的通用方法，用于定量给定细胞系或组织中特定信使核糖核酸（mRNA）的含量。我们应用这种定量PCR分析了D8基因的表达，D8是我们最近在肺癌常见缺失区域3p21染色体区域中鉴定出的一个人类基因。我们的PCR辅助检测表明，在大多数肺癌衍生的细胞系中，D8转录本的含量仅为正常肺组织中的2%或更低。该基因几乎不表达可能意味着它在肺癌发生过程中发挥了某种作用。
