远端启动子元件在人类H1.2组蛋白基因转录S期调控中的作用。
Role of a distal promoter element in the S-phase control of the human H1.2 histone gene transcription.
作者信息
Eilers A, Bouterfa H, Triebe S, Doenecke D
机构信息
Abt. Molekularbiologie, Georg-August-Universität Göttingen, Germany.
出版信息
Eur J Biochem. 1994 Jul 15;223(2):567-74. doi: 10.1111/j.1432-1033.1994.tb19026.x.
The expression of one of the human main type H1 histone genes (termed H1.2) appears to be regulated by several trans-acting factors. Upstream of consensus regulatory regions, such as the TATA-, CCAAT- and H1-box (AAACACA) sequences, a crucial control site is located between nucleotide positions -536 and -412 (relative to the ATG initiation site). Removal of this promoter portion causes in chloramphenicol acetyl transferase reporter gene constructs a loss of the S-phase control function of the H1.2 promoter in HeLa cells. Electrophoretic mobility-shift assay and DNase I footprinting analysis suggest that the H1-box variant AAACAGA is a potential control element within the distal promoter region.
人类主要的H1组蛋白基因之一（称为H1.2）的表达似乎受多种反式作用因子调控。在共有调控区上游，如TATA盒、CCAAT盒和H1盒（AAACACA）序列，一个关键的控制位点位于核苷酸位置-536和-412之间（相对于ATG起始位点）。去除该启动子部分会导致氯霉素乙酰转移酶报告基因构建体中H1.2启动子在HeLa细胞中的S期控制功能丧失。电泳迁移率变动分析和DNase I足迹分析表明，H1盒变体AAACAGA是远端启动子区域内的一个潜在控制元件。
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