一株霍乱弧菌转座子突变体的分离，该突变体具有改变的活的但不可培养的反应。
Isolation of a Vibrio cholerae transposon-mutant with an altered viable but nonculturable response.
作者信息
Ravel J, Hill R T, Colwell R R
机构信息
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202.
出版信息
FEMS Microbiol Lett. 1994 Jul 1;120(1-2):57-61. doi: 10.1111/j.1574-6968.1994.tb07007.x.
Abstract
We have isolated more than 2500 mutants of Vibrio cholerae by using transposon mutagenesis. Mutants were screened under low nutrient conditions in artificial seawater for an altered viable but nonculturable response, compared to the wild-type. Mutant JR09H1 entered the viable but nonculturable state more rapidly than the wild-type at both 25 degrees C and 4 degrees C.
摘要
我们通过转座子诱变分离出了2500多个霍乱弧菌突变体。在人工海水中的低营养条件下筛选突变体，以观察与野生型相比其活的但不可培养反应的变化。突变体JR09H1在25摄氏度和4摄氏度时进入活的但不可培养状态的速度都比野生型更快。
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