University of Maryland Biotechnology Institute, 4321 Hartwick Road, Suite 550, 20740, College Park, MD, USA.
World J Microbiol Biotechnol. 1996 Jan;12(1):28-31. doi: 10.1007/BF00327795.
Vibrio cholerae O1 can enter a state in which they remain viable but are non-culturable. Presumably, such bacteria can be pathogenic if they retain the capacity to proliferate in the human intestine following ingestion. Two groups of volunteeers were given inocula containing viable but non-culturable V. cholerae O1 of the attenuated vaccine strain CVD 101 (viable CVD 101 organisms readily colonize the human intestine). Volunteers in one of the two groups excreted viable CVD 101, demonstrating that, in the environment of the human intestine, previously non-culturable vibrios can regain the capacity to multiply. These observations support the proposition that viable but non-culturable bacterial enteropathogens may pose a potential threat to health.
霍乱弧菌 O1 可以进入一种存活但不可培养的状态。据推测，如果摄入后这些细菌仍有在人类肠道内增殖的能力，它们可能具有致病性。两组志愿者分别服用了含有减毒疫苗菌株 CVD 101 的存活但不可培养的霍乱弧菌（存活的 CVD 101 生物体很容易在人类肠道中定植）的菌苗。其中一组志愿者排出了存活的 CVD 101，表明在人类肠道环境中，以前不可培养的弧菌可以重新获得繁殖能力。这些观察结果支持了这样一种观点，即存活但不可培养的细菌病原体可能对健康构成潜在威胁。
