Banerjee-Basu S, Buonanno A
Unit on Molecular Neurobiology, National Institutes of Health, Bethesda, MD 20892.
Gene. 1994 Aug 5;145(2):241-4. doi: 10.1016/0378-1119(94)90013-2.
Troponin I is a myofibrillar protein involved in the Ca(2+)-mediated regulation of actomyosin ATPase activity. We report here the isolation and characterization of the gene coding for the slow-muscle-specific isoform of the rat troponin I polypeptide (TpnI). Using restriction mapping, PCR mapping and partial DNA sequencing, we have determined the exon/intron arrangement of this gene. The transcription unit is 10.5-kb long and contains nine exons ranging in size from 4 bp to 330 bp. The rat TpnI(slow) gene is interrupted by large intervening sequences; a 3.3-kb intron separates the 5' untranslated exons from the protein-coding exons. Comparison of the structure of rat TpnI(slow) with that of quail TpnI(fast) reveals that they have a similar intron/exon organization. The 5' untranslated region of the rat gene contains an additional exon, otherwise, the positions of introns and coding exons map to essentially identical regions in both genes.
肌钙蛋白I是一种肌原纤维蛋白，参与钙（2+）介导的肌动球蛋白ATP酶活性调节。我们在此报告大鼠肌钙蛋白I多肽（TpnI）慢肌特异性同工型编码基因的分离与特性。通过限制性图谱分析、PCR图谱分析和部分DNA测序，我们确定了该基因的外显子/内含子排列。转录单位长10.5kb，包含9个大小从4bp到330bp不等的外显子。大鼠TpnI（慢）基因被大的间隔序列打断；一个3.3kb的内含子将5'非翻译外显子与蛋白质编码外显子分开。大鼠TpnI（慢）与鹌鹑TpnI（快）的结构比较表明，它们具有相似的内含子/外显子组织。大鼠基因的5'非翻译区包含一个额外的外显子，否则，内含子和编码外显子的位置在两个基因中基本映射到相同区域。
