腺病毒心肌炎：通过对福尔马林固定、石蜡包埋组织进行基因扩增进行回顾性诊断。

Adenovirus myocarditis: retrospective diagnosis by gene amplification from formalin-fixed, paraffin-embedded tissues.

作者信息

Lozinski G M, Davis G G, Krous H F, Billman G F, Shimizu H, Burns J C

机构信息

Department of Pediatrics, UCSD School of Medicine, La Jolla 92093.

出版信息

Hum Pathol. 1994 Aug;25(8):831-4. doi: 10.1016/0046-8177(94)90255-0.

DOI:10.1016/0046-8177(94)90255-0

PMID:8056426

Abstract

Understanding the pathogenesis of viral myocarditis is linked to the availability of sensitive assays to detect viruses in clinical material. Recent advances in molecular techniques permit direct detection of viral-specific nucleic acid in tissue samples. This report describes a protocol for DNA extraction and amplification of adenovirus genome from formalin-fixed, paraffin-embedded human tissues that detects as little as 10 copies of viral genome in a background of 0.5 micrograms of human DNA. This sensitive assay permitted the examination of archived tissues to establish a retrospective diagnosis of adenoviral myocarditis in two pediatric patients.

摘要

了解病毒性心肌炎的发病机制与临床材料中检测病毒的灵敏检测方法的可用性相关。分子技术的最新进展使得能够直接在组织样本中检测病毒特异性核酸。本报告描述了一种从福尔马林固定、石蜡包埋的人体组织中提取腺病毒基因组DNA并进行扩增的方法，该方法在0.5微克人类DNA的背景下能够检测到低至10个病毒基因组拷贝。这种灵敏的检测方法使得能够对存档组织进行检查，从而对两名儿科患者的腺病毒性心肌炎做出回顾性诊断。