用于检测死后获取的福尔马林固定、石蜡包埋组织中腺病毒DNA的聚合酶链反应。

The polymerase chain reaction for detecting adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem.

作者信息

Turner P C, Bailey A S, Cooper R J, Morris D J

机构信息

Department of Pathological Sciences, Medical School, Manchester, U.K.

出版信息

J Infect. 1993 Jul;27(1):43-6. doi: 10.1016/0163-4453(93)93663-o.

DOI:10.1016/0163-4453(93)93663-o

PMID:8370944

Abstract

The polymerase chain reaction (PCR) was used to detect adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem. Adenovirus DNA was successfully amplified from specimens of lung and liver from two patients with disseminated adenovirus infection confirmed by virus isolation, electron microscopy and/or immunohistochemistry. Negative results were obtained for specimens of lung from two patients with cytomegalovirus pneumonia. The specificity of the adenovirus PCR was confirmed by means of a digoxigenin-labelled probe generated in a separate PCR. Detection of viral nucleic acid by PCR in tissues obtained post mortem has considerable diagnostic potential.

摘要

采用聚合酶链反应（PCR）检测尸检获取的福尔马林固定、石蜡包埋组织中的腺病毒DNA。通过病毒分离、电子显微镜检查和/或免疫组织化学确诊为播散性腺病毒感染的两名患者的肺和肝标本中成功扩增出腺病毒DNA。两名巨细胞病毒肺炎患者的肺标本检测结果为阴性。通过在单独的PCR中生成的地高辛标记探针证实了腺病毒PCR的特异性。在尸检获取的组织中通过PCR检测病毒核酸具有相当大的诊断潜力。