Okuda M, Umeda A, Sakai T, Ohashi T, Momoi Y, Youn H Y, Watari T, Goitsuka R, Tsujimoto H, Hasegawa A
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Int J Cancer. 1994 Aug 15;58(4):602-7. doi: 10.1002/ijc.2910580425.
Alterations of the p53 tumor-suppressor gene have been observed in a variety of human and mouse tumors. For investigation of the role of this gene in tumors of cats, feline p53 cDNA was molecularly cloned from a feline lymph-node cDNA library. The cloned cDNA (FF53) contained the whole open reading frame of p53 gene encoding 386 amino acids. The amino-acid sequence of the feline p53 gene showed 82.1% and 74.9% similarities with those of the human and mouse counterparts, respectively, and had structural characteristics in common with the p53 genes of several other species. Aberrations of the p53 gene were investigated by RT-PCR and single-strand conformation polymorphism analyses. Of 10 primary hematopoietic tumors and 3 lymphoma cell lines examined, one lymphoma and one lymphoma cell line had a point mutation of the p53 gene, resulting in single amino-acid substitutions.
在多种人类和小鼠肿瘤中都观察到了p53肿瘤抑制基因的改变。为了研究该基因在猫科动物肿瘤中的作用，从猫科动物淋巴结cDNA文库中分子克隆了猫科动物p53 cDNA。克隆的cDNA（FF53）包含编码386个氨基酸的p53基因的完整开放阅读框。猫科动物p53基因的氨基酸序列与人类和小鼠相应序列的相似性分别为82.1%和74.9%，并且与其他几个物种的p53基因具有共同的结构特征。通过RT-PCR和单链构象多态性分析研究了p53基因的畸变情况。在所检测的10例原发性造血肿瘤和3个淋巴瘤细胞系中，1例淋巴瘤和1个淋巴瘤细胞系存在p53基因的点突变，导致单个氨基酸替换。
