Mayr B, Reifinger M, Alton K, Schaffner G
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Vet Res Commun. 1998 Jun;22(4):249-55. doi: 10.1023/a:1006091215330.
Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG-->GAG, lysine-->glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.
对20例猫肿瘤进行了外显子5至8区域的测序，以检测肿瘤抑制基因p53的突变情况。在一例膀胱梭形细胞肉瘤中，检测到外显子5中的一个错义突变（密码子164 AAG→GAG，赖氨酸→谷氨酸）。在一例多形性肉瘤中，观察到一个23 bp的缺失，涉及内含子5和外显子6之间的剪接位点。在一例纤维肉瘤中，发现p53有一个6 bp的缺失，覆盖外显子7的2 bp和内含子7的4 bp，包括剪接位点。该研究在猫的不同类型肉瘤中发现了三种新的p53突变。
