Fernandez-Salas I, Rodriguez M H, Roberts D R
Centro de Investigación de Paludismo, Chiapas, Mexico.
J Med Entomol. 1994 May;31(3):340-7. doi: 10.1093/jmedent/31.3.340.
Mark-release-recapture experiments were conducted to determine the length of the gonotrophic cycle and survivorship of Anopheles pseudopunctipennis Theobald in the Tapachula foothills of southern Mexico. Separate trials with wild-caught females were conducted in the early and late dry season to examine intraseasonal differences. The gonotrophic cycle of insectary-reared, nulliparous females was estimated during the late dry season. A total of 5.4-5.7% of marked females was recaptured. A 3-d gonotrophic cycle was characteristic of wild-caught females, whereas a 4-d gonotrophic cycle was characteristic for insectary-reared females. Engorged, wild-caught females completed oogenesis in approximately 60 h under field conditions. Blood digestion in 23.5% of 2,221 and 21.9% of 1,195 engorged, wild-caught females held in the insectary was not accompanied by egg development. Dissections of these females showed that nearly 85% were nulliparous and presumably pregravid. These statistics plus other field data were used to determine that 60% of all nulliparous entered a pregravid condition. The body size and wing length of gravids was significantly larger than pregravids. Daily survival estimates of 0.875 and 0.884 were calculated for field-collected females during the early and late dry season, respectively.
为了确定墨西哥南部塔帕丘拉山麓地区伪点按蚊的生殖营养周期长度和存活率，开展了标记-释放-再捕获实验。在旱季早期和晚期分别对野外捕获的雌蚊进行了单独试验，以研究季节内差异。在旱季晚期对饲养在昆虫饲养室的未产卵雌蚊的生殖营养周期进行了估计。总共5.4%-5.7%的标记雌蚊被重新捕获。野外捕获的雌蚊的生殖营养周期为3天，而饲养在昆虫饲养室的雌蚊的生殖营养周期为4天。在野外条件下，饱血的野外捕获雌蚊大约在60小时内完成卵子发生。在昆虫饲养室饲养的2221只饱血野外捕获雌蚊中，23.5%以及1195只饱血野外捕获雌蚊中21.9%的血液消化过程未伴随卵子发育。对这些雌蚊进行解剖发现，近85%为未产卵雌蚊，推测处于妊娠前期。这些统计数据以及其他野外数据被用于确定所有未产卵雌蚊中有60%进入了妊娠前期状态。妊娠雌蚊的体型和翅长显著大于妊娠前期雌蚊。分别计算出旱季早期和晚期野外采集雌蚊的每日存活率估计值为0.875和0.884。
