Collins F M, Auclair L, Montalbine V
Int Arch Allergy Appl Immunol. 1975;48(5):680-90. doi: 10.1159/000231355.
The growth of Mycobacterium bovis (BCG Montreal) and M. tuberculosis Erdman was determined in normal and T-cell depleted (THXB) mice when injected subcutaneously into a hind footpad. The bacilli multiplied only to a limited extent within the footpad itself but the infection quickly spread to the draining popliteal lymph node to eventually reach the liver, spleen and lung. The amount of systemic growth seen in the THXB mice was 10-100 times greater than in the normal controls, all of which developed a tuberculin hypersensitivity and an immune response in 14-18 days. T-cell depletion completely inhibited the expression of tuberculin sensitivity by the infected host as well as ablating the antituberculous response against both the vaccinating BCG population and a superinfecting Erdman challenge inoculum. Incorporation studies in the THXB mice indicated a striking reduction in cell division within the draining lymph node but there was an unexpected elevation in the level of incorporation by the lung cells as the BCG infection progressed. The significance of these findings is discussed in relation to the possible use of the BCG footpad model for studies of leprosy immunity.
将牛分枝杆菌（卡介苗蒙特利尔株）和结核分枝杆菌埃尔德曼株皮下注射到正常小鼠和T细胞耗竭（THXB）小鼠的后足垫中，测定它们的生长情况。杆菌在足垫内仅有限增殖，但感染迅速扩散至引流的腘淋巴结，最终累及肝脏、脾脏和肺脏。THXB小鼠中观察到的全身生长量比正常对照大10至100倍，所有正常对照在14至18天内均产生结核菌素超敏反应和免疫应答。T细胞耗竭完全抑制了受感染宿主结核菌素敏感性的表达，同时消除了针对接种的卡介苗群体和超感染的埃尔德曼攻击接种物的抗结核应答。在THXB小鼠中的掺入研究表明，引流淋巴结内的细胞分裂显著减少，但随着卡介苗感染的进展，肺细胞的掺入水平意外升高。结合麻风病免疫研究中卡介苗足垫模型的可能用途，讨论了这些发现的意义。
