Stieritz D D, Holder I A
J Infect Dis. 1975 Jun;131(6):688-91. doi: 10.1093/infdis/131.6.688.
An experimental burned mouse model is described that is clinically relevant to burn wound sepsis caused by Pseudomonas aeruginosa. Mice subjected to a nonlethal burn by flame were challenged with P. aeruginosa. The LD50 after subcutaneous injection in the skin of the burn up to 24 hr after the burn was smaller than 10 organisms vs. 10-6 organisms in normal animals. By three days after the burn, the value returned to and exceeded that of normal animals. This dramatic change in the LD50 after the burn was not seen when mice were challenged with other organisms. Challenge with P. aeruginosa by different routes immediately after burning showed less dramatic decreases in the LD50. Enumeration of infecting organisms in the skin of the burn and in major organs suggests the possibility of a toxic event.
描述了一种实验性烧伤小鼠模型，该模型与铜绿假单胞菌引起的烧伤创面脓毒症具有临床相关性。通过火焰对小鼠造成非致死性烧伤，然后用铜绿假单胞菌进行攻击。烧伤后24小时内在烧伤皮肤皮下注射的半数致死量（LD50）小于10个菌，而正常动物为10^6个菌。烧伤后三天，该值恢复并超过正常动物的值。当用其他微生物攻击小鼠时，未观察到烧伤后LD50的这种显著变化。烧伤后立即通过不同途径用铜绿假单胞菌攻击，LD50的下降不太显著。对烧伤皮肤和主要器官中的感染菌进行计数表明存在毒性事件的可能性。
