Goldberg D E, Stuart J, Koerner F C
Bouvé College of Pharmacy and Health Sciences, Northeastern University, Boston, Massachusetts.
Mod Pathol. 1994 Apr;7(3):401-6.
We sought to establish an easily interpretable and highly sensitive immunohistochemical method for detecting progesterone receptor protein in formalin-fixed, paraffin-embedded samples of breast carcinomas. Beginning with a conventional immunoperoxidase staining procedure, we incorporated microwave heating to compensate for the effects of conventional fixation and processing and then applied the method to 90 samples of primary breast carcinomas. We used the results of hormone binding assays as true values to establish the sensitivity, specificity, positive predictive value, and negative predictive value of the staining method. Our technique yielded good preservation of morphological detail and low nonspecific staining of background tissue. Comparisons of the results of staining and biochemical assays revealed that this progesterone receptor immunostaining procedure shows high sensitivity and acceptable specificity. We believe its performance characteristics make reliable study of small specimens and archival material possible.
我们试图建立一种易于解释且高度灵敏的免疫组织化学方法，用于检测福尔马林固定、石蜡包埋的乳腺癌样本中的孕激素受体蛋白。从传统的免疫过氧化物酶染色程序开始，我们采用微波加热来弥补传统固定和处理的影响，然后将该方法应用于90例原发性乳腺癌样本。我们将激素结合试验的结果作为真值，以确定染色方法的敏感性、特异性、阳性预测值和阴性预测值。我们的技术能很好地保存形态细节，背景组织的非特异性染色较低。染色结果与生化检测结果的比较表明，这种孕激素受体免疫染色程序具有高敏感性和可接受的特异性。我们认为其性能特点使对小标本和存档材料进行可靠研究成为可能。
