Katoh A K, Stemmler N, Specht S, D'Amico F
Department of Laboratory Medicine, Mercy Hospital, Pittsburgh, Pennsylvania 15219, USA.
Biotech Histochem. 1997 Nov;72(6):291-8. doi: 10.3109/10520299709096524.
Immunoperoxidase staining was performed for estrogen and progesterone receptors in 93 cases of primary breast carcinoma. Breast tumor samples were fixed in formalin and embedded in paraffin. Antigen retrieval was performed by microwave heating in citrate buffer, pH 6.0, using precisely defined and reproducible conditions. The cases studied included material from the current year and from paraffin blocks retrieved from archival storage dating back to 1981. In all cases, estrogen and progesterone receptor values determined by biochemical assay were available for comparison with the immunohistochemical results. We found 94% agreement of results between the two methods.
对93例原发性乳腺癌进行了雌激素和孕激素受体的免疫过氧化物酶染色。乳腺肿瘤样本用福尔马林固定并石蜡包埋。使用精确界定且可重复的条件，通过在pH 6.0的柠檬酸盐缓冲液中微波加热进行抗原修复。所研究的病例包括当年的材料以及从可追溯至1981年的存档石蜡块中获取的材料。在所有病例中，均可获得通过生化测定确定的雌激素和孕激素受体值，以便与免疫组化结果进行比较。我们发现两种方法的结果一致性为94%。
