Marcot I, Migeon C, Parache R M, Marchal S, Virion J M
Laboratoire d'anatomie et de cytologie pathologiques, Center Alexis-Vautrin (CRLCC), Vandoeuvre-lès-Nancy, France.
Bull Cancer. 1997 Jun;84(6):613-8.
As the biochemical assay, the measurement of hormone receptors by immunocytochemistry in invasive breast cancers may predict the ability of patients to respond to hormone therapy. The objective of this work is to determine the reliability of hormone receptors analysis on cytologic spreadings. Estrogen and progesterone receptors analysis was carried out in 109 invasive breast carcinomas: (1) with a SAMBA 2005 image analysis system on frozen cytologic spreadings (ER/PR-ICA, Abbott); (2) by visual evaluation on paraffin sections (ER-1D5, Dako; PR-ICA, Abbott); (3) by biochemistry (EIA, Abbott). There is a significant correlation between the three methods of hormone receptors analysis (0.716 to 0.842). The sensitivity of immunocytochemical methods ranges from 88.0 to 94.3%, and the specificity from 70.0 to 94.7%. The minimum concordance is 87.2%. This study demonstrates that immunocytochemistry of hormone receptors is a good alternative to biochemical measurement, especially when applied to cytologic spreadings. Therefore, immunocytochemistry can be used, when conventional biochemical assay cannot be performed for hormone receptors evaluation, particularly on cytoponctions.
作为生化检测方法，通过免疫细胞化学对浸润性乳腺癌中的激素受体进行检测，可能预测患者对激素治疗的反应能力。本研究的目的是确定激素受体分析在细胞涂片上的可靠性。对109例浸润性乳腺癌进行了雌激素和孕激素受体分析：（1）使用SAMBA 2005图像分析系统对冰冻细胞涂片进行分析（ER/PR-ICA，雅培公司）；（2）通过对石蜡切片进行视觉评估（ER-1D5，达科公司；PR-ICA，雅培公司）；（3）通过生化方法（酶免疫分析，雅培公司）。三种激素受体分析方法之间存在显著相关性（0.716至0.842）。免疫细胞化学方法的敏感性范围为88.0%至94.3%，特异性范围为70.0%至94.7%。最低一致性为87.2%。本研究表明，激素受体的免疫细胞化学是生化检测的良好替代方法，特别是应用于细胞涂片时。因此，当无法进行常规生化检测来评估激素受体时，尤其是在细胞穿刺时，可使用免疫细胞化学方法。
