在浮萍（Lemna gibba L. G-3）中受光敏色素作用负调控的NPR基因，也可受脱落酸正调控。
NPR genes, which are negatively regulated by phytochrome action in Lemna gibba L. G-3, can also be positively regulated by abscisic acid.
作者信息
Williams S A, Weatherwax S C, Bray E A, Tobin E M
机构信息
Department of Biology, University of California, Los Angeles 90024-1606.
出版信息
Plant Physiol. 1994 Jul;105(3):949-54. doi: 10.1104/pp.105.3.949.
Abstract
We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response to concentrations as low as 10 nM. We have also tested phytochrome responsiveness of 5' promoter-deletion constructs of one of these genes, NPR1, in transient assays utilizing particle bombardment. This analysis demonstrated that DNA sequences important for phytochrome regulation are present downstream of -198 from the transcription start site. A response to ABA treatment could also be observed in the transient assay system. When intact plants were placed in darkness, there was an increase in ABA levels as well as increased levels of NPR1 and NPR2 RNA.
摘要
我们发现，来自浮萍（Lemna gibba L. G-3）的两个基因NPR1和NPR2可受光敏色素作用的负调控，也可受植物激素脱落酸（ABA）的正调控。这两个基因对外源ABA的低浓度均有响应；在低至10 nM的浓度下，就能检测到NPR1 RNA的增加。我们还利用微粒轰击法在瞬时分析中测试了其中一个基因NPR1的5'启动子缺失构建体的光敏色素反应性。该分析表明，对光敏色素调控至关重要的DNA序列存在于转录起始位点-198下游。在瞬时分析系统中也能观察到对ABA处理的反应。当完整植株置于黑暗中时，ABA水平会升高，NPR1和NPR2 RNA的水平也会升高。
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