Marshall B E, Clarke W R, Costarino A T, Chen L, Miller F, Marshall C
Center for Anesthesia Research, Hospital of the University of Pennsylvania, Philadelphia 19104.
Respir Physiol. 1994 May;96(2-3):231-47. doi: 10.1016/0034-5687(94)90129-5.
In 12 pentobarbital anesthetized dogs the lungs were independently ventilated with a double piston ventilator. The right lung was ventilated throughout with 100% oxygen. Blood was drawn from the right atrium and pumped through a bubble oxygenator to a cannula in the ligated left main pulmonary artery. The pressures in the left main pulmonary artery and the left atrium were recorded during constant flow while the oxygen tension in the left lung alveolar gas and the perfusate were varied either to match each other (Protocol 1) or differ (Protocol 2) over the range from "zero" to "100%" oxygen. From the combined data a three dimensional response surface for hypoxic pulmonary vasoconstriction was derived. The maximum increase of pulmonary vascular resistance (r%PVRmax) was defined at a stimulus oxygen tension (PSO2) of 10 mmHg amounting to a 3.15 +/- (0.18)-fold increase of the vascular resistance on "100%" oxygen. The stimulus oxygen tension was shown to be PSO2 = PVO2(0.41) x PAO2(0.59) and the dose-response sigmoid for hypoxic pulmonary vasoconstriction in canine lungs was derived as r%PVRmax = 100 (PSO2(-2.616))/(6.683 x 10(-5) + PSO2(-2.616)) These results appear to reconcile observations from a number of laboratories and to be of quite general application.
在12只戊巴比妥麻醉的犬中，用双活塞呼吸机对肺进行独立通气。右肺全程用100%氧气通气。从右心房取血，通过鼓泡式氧合器泵入结扎的左主肺动脉内的插管。在恒定流量过程中记录左主肺动脉和左心房的压力，同时在“零”至“100%”氧气范围内，使左肺肺泡气和灌注液中的氧张力相互匹配（方案1）或不同（方案2）。根据合并的数据得出了缺氧性肺血管收缩的三维反应面。肺血管阻力的最大增加（r%PVRmax）定义为刺激氧张力（PSO2）为10 mmHg时，相当于“100%”氧气时血管阻力增加3.15±（0.18）倍。刺激氧张力显示为PSO2 = PVO2(0.41)×PAO2(0.59)，犬肺缺氧性肺血管收缩的剂量反应S形曲线推导为r%PVRmax = 100 (PSO2(-2.616))/(6.683×10(-5) + PSO2(-2.616)) 这些结果似乎与许多实验室的观察结果一致，且具有相当广泛的应用。
