O'Brien W D, Zachary J F
Department of Electrical and Computer Engineering, University of Illinois, Urbana 61801.
Ultrasound Med Biol. 1994;20(3):299-307. doi: 10.1016/0301-5629(94)90070-1.
Twenty-four mice and sixteen rabbits were evaluated at one exposure duration (10 min) and at three exposure acoustic pressure levels (0, 100 and 145 kPa) at an ultrasonic frequency of 30 kHz, continuous wave for the purpose of testing whether there was a species difference in the degree of sensitivity to ultrasound-induced lung damage. This study was undertaken because it was hypothesized that the mouse may not be an acceptable or suitable animal model for studies that examine the effects of ultrasound on lung tissue for purposes of extrapolating or estimating the degree of potential damage in other species. The rabbit was selected for comparison to the mouse because the rabbit exhibited sufficient physiological and morphological differences from those of the mouse to test this hypothesis. Using exactly the same exposure conditions and lung assessment criteria, it appeared that the mouse lung was more sensitive to ultrasound-induced damage than that of the rabbit by a factor of between 2.8 and 3.6. Lung lesions in mice and rabbits were similar in character, but were much more severe and extensive in mice. Lesions in both species consisted of intraalveolar hemorrhage that appeared as dark red to red-black areas that were visible on the pleural surfaces and that extend within the lung parenchyma.
在超声频率为30kHz、连续波的条件下，对24只小鼠和16只兔子进行了评估，暴露持续时间为10分钟，暴露声压水平分为三个等级（0、100和145kPa），目的是测试对超声诱导的肺损伤的敏感程度是否存在物种差异。进行这项研究是因为有这样的假设：对于研究超声对肺组织的影响以推断或估计其他物种潜在损伤程度的研究而言，小鼠可能不是一个可接受或合适的动物模型。选择兔子与小鼠进行比较，是因为兔子与小鼠在生理和形态上表现出足够的差异，以检验这一假设。使用完全相同的暴露条件和肺部评估标准，结果显示小鼠肺对超声诱导损伤的敏感性比兔子高2.8至3.6倍。小鼠和兔子的肺部病变特征相似，但小鼠的病变更为严重和广泛。两个物种的病变均包括肺泡内出血，表现为暗红色至红黑色区域，在胸膜表面可见，并延伸至肺实质内。
