用表皮生长因子对胚胎进行体外处理可提高活力，并增加移植到受体小鼠体内的囊胚的着床率。
In vitro treatment of embryos with epidermal growth factor improves viability and increases the implantation rate of blastocysts transferred to recipient mice.
作者信息
Morita Y, Tsutsumi O, Taketani Y
机构信息
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Am J Obstet Gynecol. 1994 Aug;171(2):406-9. doi: 10.1016/s0002-9378(94)70042-7.
OBJECTIVE
Our purpose was to study, by means of a mouse embryo donation model, the possible role of epidermal growth factor in the development of preimplantation embryos.
STUDY DESIGN
Seven blastocysts developed either in vivo or in vitro, with or without treatment with epidermal growth factor, were transferred to a uterine horn of pseudopregnant recipient mice on day 3 (the presence of a vaginal plug indicated day 1 of pseudopregnancy). Recipients were killed on day 8 of pseudopregnancy, and the number of implantation sites was counted.
RESULTS
The implantation rate in recipient mice of in vitro-cultured blastocysts was significantly lower than that of embryos grown in vivo (i.e., 57.1% vs 84.2%) (p < 0.001). Treatment of embryos in vitro with epidermal growth factor, 10 ng/ml, effectively prevented a decrease in implantation rate (77.9%).
CONCLUSION
Epidermal growth factor may play a stimulatory role in the development of the preimplantation mouse embryo.
目的
我们旨在通过小鼠胚胎捐赠模型研究表皮生长因子在植入前胚胎发育中的可能作用。
研究设计
7个在体内或体外发育的囊胚，无论是否用表皮生长因子处理，在第3天被移植到假孕受体小鼠的子宫角（阴道栓的出现表明假孕第1天）。在假孕第8天处死受体，计数着床部位的数量。
结果
体外培养的囊胚在受体小鼠中的着床率显著低于体内生长的胚胎（即57.1%对84.2%）（p<0.001）。用10 ng/ml表皮生长因子体外处理胚胎可有效防止着床率下降（77.9%）。
结论
表皮生长因子可能在植入前小鼠胚胎发育中起刺激作用。
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