Hegele R G, Bicknell S G, Bailey D J, Cameron R G
University of British Columbia Pulmonary Research Laboratory, St Paul's Hospital, Vancouver, Canada.
Arch Pathol Lab Med. 1994 Aug;118(8):795-6.
We report a case of posttransplant lymphoproliferative disorder diagnosed within 4 weeks of orthotopic liver transplantation from a male donor to a female recipient. To determine whether the posttransplant lymphoproliferative disorder was of donor or recipient origin, nonisotopic in situ hybridization for the human Y chromosome was performed on formalin-fixed, paraffin-embedded sections of the donor liver using a digoxigenin-labeled probe. The lymphoid cells hybridized with the Y chromosome probe, indicative of a male genotype consistent with posttransplant lymphoproliferative disorder of donor origin. This case illustrates that nonisotopic in situ hybridization for the Y chromosome can discriminate between donor and recipient cells in sex-mismatched organ transplants.
我们报告了一例原位肝移植术后4周内诊断出的移植后淋巴细胞增生性疾病，供体为男性，受体为女性。为确定该移植后淋巴细胞增生性疾病是供体来源还是受体来源，使用地高辛标记的探针，对供体肝脏经福尔马林固定、石蜡包埋的切片进行人Y染色体的非同位素原位杂交。淋巴样细胞与Y染色体探针杂交，表明男性基因型，符合供体来源的移植后淋巴细胞增生性疾病。该病例说明，Y染色体的非同位素原位杂交可在性别不匹配的器官移植中区分供体细胞和受体细胞。
