Le Frère-Belda M A, Martin N, Gaulard P, Zafrani E S
Département de Pathologie and Equipe Recommandeé 270, Université Paris XII-Val de Marne, Hôpital Henri Mondor, Créteil, France.
Mod Pathol. 1997 Jul;10(7):701-7.
Post-transplantation lymphoproliferative disorders (PTLDs) have been reported after bone marrow and solid-organ transplantation. It might be of interest to know the origin of PTLDs, because it has been suggested that a donor origin could be related to a better prognosis. We studied five cases of PTLD in sex-mismatched allografted recipients by in situ hybridization technique for chromosome Y on isolated cells as well as on frozen and on routinely fixed and paraffin-embedded material. Two proved to be of donor origin, including the only case of PTLD arising in the graft, and three of recipient origin. The best results were obtained on isolated cells, but it must be emphasized that hybridization on tissue sections from frozen material or from material fixed in formalin or in formalin-acetic acid-alcohol and then paraffin embedded also gave good results. Hybridization after fixation in Bouin's liquid was not reliable. These results suggest that evaluation of the origin of PTLDs can easily be performed on routinely processed cytologic and histologic material.
骨髓移植和实体器官移植后均有移植后淋巴细胞增生性疾病（PTLD）的报道。了解PTLD的起源可能会有意义，因为有观点认为供体起源可能与较好的预后相关。我们通过原位杂交技术，对5例性别不匹配的同种异体移植受者的PTLD病例进行了研究，该技术用于检测分离细胞以及冷冻、常规固定和石蜡包埋材料中的Y染色体。其中2例证明是供体起源，包括唯一一例发生在移植物中的PTLD病例，另外3例是受体起源。在分离细胞上获得了最佳结果，但必须强调的是，对冷冻材料或用福尔马林、福尔马林 - 乙酸 - 酒精固定后再石蜡包埋的组织切片进行杂交，也能得到良好结果。用Bouin液固定后进行杂交不可靠。这些结果表明，对PTLD起源的评估可以很容易地在常规处理的细胞学和组织学材料上进行。
