Zhang Y, Morgan T
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Am J Hypertens. 1994 May;7(5):448-52. doi: 10.1093/ajh/7.5.448.
The secretion and synthesis of renin were studied in mice by measuring aortic, right and left renal vein renin, renal renin, and mRNA for renin. The role of the macula densa was evaluated in a kidney made hydronephrotic by tying the ureter 6 weeks earlier. There was no net secretion of renin from the left hydronephrotic kidney even when the mice were placed in a high secretory state by sodium restriction or enalapril. Sodium restriction and enalapril increased renin content to a similar extent in the normal and hydronephrotic kidney. High sodium intake decreased renin content in the normal and hydronephrotic kidney and also decreased the enalapril-stimulated renin content. Changes in mRNA in the same direction to renal renin implied that this was due to increased synthesis. Thus secretion and synthesis of renin can be disassociated. The macula densa is important for renin secretion but not for the stimulation of synthesis.
通过测量主动脉、右肾静脉和左肾静脉肾素、肾组织肾素以及肾素mRNA，研究了小鼠肾素的分泌和合成。通过6周前结扎输尿管使肾脏积水，以此评估致密斑的作用。即使通过限制钠摄入或给予依那普利使小鼠处于高分泌状态，左肾积水的肾脏也没有肾素的净分泌。限制钠摄入和依那普利使正常肾脏和积水肾脏中的肾素含量增加到相似程度。高钠摄入降低了正常肾脏和积水肾脏中的肾素含量，也降低了依那普利刺激后的肾素含量。与肾组织肾素变化方向相同的mRNA变化表明，这是由于合成增加所致。因此，肾素的分泌和合成可能是分离的。致密斑对肾素分泌很重要，但对合成的刺激不重要。
