Shakibaei M, Milaninezhad M, Risse H J
Institut für Anatomie, Freie Universität Berlin, Germany.
J Struct Biol. 1994 Mar-Apr;112(2):125-35. doi: 10.1006/jsbi.1994.1014.
Bloodstream forms of Trypanosoma congolense were cocultivated in vitro with vascular endothelial cells. The trypanosomes adhere specifically to the endothelial surfaces of the anterior part of their flagella, as shown by scanning and transmission electron microscopy. The interaction between parasite and host cell is very tight, and frequently the accumulation of endocytotic vesicles near the contact site is observed. Immunoelectron microscopy revealed a compound distributed over the total surface of the trypanosomes and reacting with antibodies against the beta 1 integrin chain, but no reaction was found with anti-alpha 1 or anti-alpha 2 antibodies. Integrins are typical adhesion molecules and are now shown to be present at the surface of T. congolense by electron microscopy and by immunofluorescence. A direct participation of this substance in the specific adhesion to endothelium, however, could not be proven.
将刚果锥虫的血流型与血管内皮细胞进行体外共培养。扫描电子显微镜和透射电子显微镜显示，锥虫特异性地粘附在其鞭毛前部的内皮表面。寄生虫与宿主细胞之间的相互作用非常紧密，并且经常观察到接触部位附近有内吞小泡的积累。免疫电子显微镜显示一种化合物分布在锥虫的整个表面，并与抗β1整合素链的抗体发生反应，但未发现与抗α1或抗α2抗体发生反应。整合素是典型的粘附分子，现在通过电子显微镜和免疫荧光显示其存在于刚果锥虫的表面。然而，无法证明这种物质直接参与了对内皮的特异性粘附。
