Roughley P J, Melching L I, Recklies A D
Genetics Unit, Shriners Hospital for Crippled Children, Department of Surgery, McGill University, Montreal, Canada.
Matrix Biol. 1994 Jan;14(1):51-9. doi: 10.1016/0945-053x(94)90029-9.
In chondrocytes isolated directly from human articular cartilage, without subsequent culture, biglycan mRNA levels decreased with the age of the donor, whereas those for decorin increased. In cultured chondrocytes in the presence of FCS, mRNA levels for biglycan remained similar to those in uncultured counterparts, while decorin transcription became depressed. The differential effect on decorin and biglycan mRNA expression was mimicked by the addition of TGF-beta. In the absence of exogenously added growth factors (TGF-beta or FCS) biglycan mRNA levels decreased, while those for decorin increased. In contrast, IGF-I showed no differential modulation of the relative abundance of the two messages. The opposite regulation of these two proteoglycans by TGF-beta was also reflected at the level of protein synthesis. It would appear that the need of articular cartilage for decorin is greatest in the adult, whereas the need for biglycan is greatest in the juvenile.
在直接从人关节软骨分离且未经后续培养的软骨细胞中，双糖链蛋白聚糖的mRNA水平随供体年龄增长而降低，而核心蛋白聚糖的mRNA水平则升高。在含有胎牛血清（FCS）的培养软骨细胞中，双糖链蛋白聚糖的mRNA水平与未培养的对应细胞相似，而核心蛋白聚糖的转录则受到抑制。添加转化生长因子-β（TGF-β）可模拟对核心蛋白聚糖和双糖链蛋白聚糖mRNA表达的不同影响。在没有外源添加生长因子（TGF-β或FCS）的情况下，双糖链蛋白聚糖的mRNA水平降低，而核心蛋白聚糖的mRNA水平升高。相反，胰岛素样生长因子-I（IGF-I）对这两种信息分子的相对丰度没有差异调节作用。TGF-β对这两种蛋白聚糖的相反调节也反映在蛋白质合成水平上。似乎关节软骨对核心蛋白聚糖的需求在成年人中最大，而对双糖链蛋白聚糖的需求在青少年中最大。
