Demoor-Fossard M, Redini F, Boittin M, Pujol J P
Laboratoire de Biochimie du Tissu Conjonctif, Faculté de Médecine, Caen, France.
Biochim Biophys Acta. 1998 Jun 16;1398(2):179-91. doi: 10.1016/s0167-4781(98)00044-x.
In this study, the levels of mRNAs coding for aggrecan, decorin and biglycan in rabbit articular chondrocytes were investigated, using both monolayer and 3D-alginate cultures treated with TGF-beta 1 and IL-1 beta. The cells were shown to express higher amounts of proteoglycan messages, specially the aggrecan, in gels than in monolayers. TGF-beta 1 increased aggrecan mRNA in both systems, whereas biglycan message was elevated only in alginate. It markedly decreased decorin expression in monolayer, either in primary or passaged cultures. In contrast, IL-1 beta had a weak inhibitory effect on both decorin and biglycan expression. Subculturing induced a dramatic decrease of aggrecan mRNA, while that of decorin augmented. Biglycan expression transiently increased after two passages, whereas it declined in further subcultures. Passaged chondrocytes transferred to alginate re-expressed high levels of aggrecan, decorin and biglycan. The data point to the influence of morphology, proliferative state and environment of the articular chondrocytes on their biosynthetic responses to cytokines. Although these immature cells do not fully reflect the adult chondrocytes present in the cartilage, this study may help in understanding the behaviour of these cells in osteoarticular diseases, where the surrounding extracellular matrix is profoundly altered.
在本研究中，利用经转化生长因子-β1（TGF-β1）和白细胞介素-1β（IL-1β）处理的单层培养和3D海藻酸盐培养，研究了兔关节软骨细胞中编码聚集蛋白聚糖、核心蛋白聚糖和双糖链蛋白聚糖的mRNA水平。结果显示，与单层培养相比，细胞在凝胶中表达更高量的蛋白聚糖信息，特别是聚集蛋白聚糖。TGF-β1在两个系统中均增加了聚集蛋白聚糖mRNA的表达，而双糖链蛋白聚糖的信息仅在海藻酸盐中升高。它显著降低了单层培养（原代培养或传代培养）中核心蛋白聚糖的表达。相反，IL-1β对核心蛋白聚糖和双糖链蛋白聚糖的表达均有微弱的抑制作用。传代培养导致聚集蛋白聚糖mRNA急剧下降，而核心蛋白聚糖的mRNA增加。双糖链蛋白聚糖的表达在传代两次后短暂增加，而在进一步传代培养中下降。转移至海藻酸盐中的传代软骨细胞重新表达高水平的聚集蛋白聚糖、核心蛋白聚糖和双糖链蛋白聚糖。这些数据表明关节软骨细胞的形态、增殖状态和环境对其对细胞因子的生物合成反应有影响。尽管这些未成熟细胞不能完全反映软骨中存在的成年软骨细胞，但本研究可能有助于理解这些细胞在骨关节疾病中的行为，在这些疾病中周围的细胞外基质发生了深刻改变。
