Mizuno Y, Hosoi T, Inoue S, Ikegami A, Kaneki M, Akedo Y, Nakamura T, Ouchi Y, Chang C, Orimo H
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Calcif Tissue Int. 1994 Apr;54(4):325-6. doi: 10.1007/BF00295958.
Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
采用免疫细胞化学技术检测雄激素受体（AR）在小鼠破骨细胞样多核细胞（OCs）中的表达。通过将小鼠成骨细胞与骨髓细胞共培养获得小鼠OCs。实验使用了三种在兔体内针对人AR不同部位产生的多克隆抗AR抗体。在小鼠OCs的细胞核和核周区域观察到了AR的特异性染色。这是首次报道在破骨细胞样细胞中存在AR。
